Arts, Literature & Entertainment
- Jon Acuff, author of 2013 New York Times bestseller Start
- Mary B. Anderson, actor, Gone with the Wind, Lifeboat
- Zane Birdwell, Grammy Award–winning recording engineer
- James Briscione, award winning chef and champion of the first season of Chopped
- Kate Campbell, Nashville-based singer-songwriter
- Jennifer Pharr Davis, world-record holder, fastest through hike of the Appalachian Trial (2011)
- Karen Fairchild and Kimberly Schlapman, Grammy Award–winning vocalists for Little Big Town
- Wayne Flynt, Pulitzer Prize–nominated historian
- Elizabeth Futral, international opera star
- Anne George, Pulitzer Prize–nominated poet and Agatha Award–winning mystery novelist
- Tony Hale, Emmy Award–winning actor
- Harold Martin, Pulitzer Prize–winning journalist (Montgomery Advertiser, 1970)
- Susan Patterson, international opera star
- Sarah Smiley, nationally syndicated columnist and author
- Kristian Paul Stanfill, Christian rock singer-songwriter and Dove Award nominee
- Perry Stephens, actor, Loving, The Bold and the Beautiful
- Jay and Katherine Wolf, renowned authors and public speakers
Business
- Daryl Byrd, former president and chief executive officer, IberiaBank
- Don Cathy, executive vice president, Chick-fil-A, president, Dwarf House Inc.
- Stephen Christopher, former president/chief executive officer, ING Life Insurance
- Gary Cooney, vice-chairman of McGriff, Seibels & Williams
- Gayla Delly, former president and chief executive officer, Benchmark
- Andrew Gerow Hodges, former executive vice president, Liberty National Life Insurance Company; received the Salvation Army's national William Booth Award, decorated with two Bronze Stars; former president of Operation New Birmingham
- James F. House, president and chief executive officer, First United Security Bank and United Security Bancshares Inc.
- Thomas Luckie, chairman and chief executive officer, Luckie & Co.
- R. Clayton McWhorter, former president and chief executive officer of the Hospital Corporation of America
- Marvin Mann, founding chairman and chairman emeritus, Lexmark; former president and chief executive officer of the Satellite Business Systems Company
- Mickey Newsome, chairman and founding chief executive officer, Hibbett Sports Inc.
- William S. Propst Sr., founder and former president, Qualitest/Vintage Pharmaceuticals; founder of Propst Properties
- Leigh Ann Pusey, former president and chief executive officer, American Insurance Association; executive vice president, Eli Lilly and Company; deputy assistant to President George Bush for public liaison
- Jimmy Rane, president and chief executive officer, Great Southern Wood Preserving
- William Stevens, former chairman and chief executive officer, Motion Industries
Education
- Hugh C. Bailey, former president, Valdosta State University (1978–2002)
- J. Claude Bennett, former president, University of Alabama at Birmingham (1993–96)
- Mary Lynne Capilouto, former dean, University of Alabama at Birmingham School of Dentistry (1998–2005)
- Sonia Carrington, National Distinguished Principal of the Year (2008)
- Gwen Childs, Milken Family Foundation National Educator Award (2002)
- Kirby Godsey, former president, Mercer University (1979–2006)
- William E. Hull, former provost, Southern Seminary (1972–75) and Samford University (1987–96)
- Heather Hurt, Milken Family Foundation National Educator Award (2018)
- Jasper Hutto, founder, South’s first Department of Journalism (Samford, 1915)
- David Gordon Lyon, former Hollis Chair, Harvard Divinity School (1882–1910)
- Ladell Payne, former president, Randolph Macon College (1979–97)
- Betsy Rogers, National Teacher of the Year (2003), Alabama State Teacher of the Year (2002)
- Pamela Smith, Milken Family Foundation National Educator Award (2012)
Health
- John G. Beard, chairman and president, Alacare Home Health & Hospice
- Peter J. Clemens IV, executive vice president and chief financial officer, Surgical Care Affiliates
- Francois J. Coutu, president and chief executive officer, the Jean Coutu Group, Montreal, Quebec, Canada
- Juanzetta S. Flowers, pioneer, nurse practitioner movement
- Frances Watts Johnston, pioneer, surgical technology
- John B. McWhorter, president, Baylor University Medical Center at Dallas
- Ida V. Moffett, nursing pioneer and namesake of Samford’s nursing school
- Sylvia Rayfield, internationally known nurse educator, entrepreneur and business leader
- James E. Shmerling, president and chief executive officer, Children’s Hospital, Denver, Colorado
- Connie Yarbro, internationally known leader and lecturer, oncology nursing
- Sylvia Young, president and chief executive officer, HealthONE and HCA’s Continental Division
Law & Politics
- Robert B. Aderholt, U.S. House of Representatives (Alabama, 1997 – present)
- Karon Bowdre, chief judge, U.S. District Court, Northern District of Alabama (2013-2019); currently has senior status (2020-present) and is chair of Samford University’s Board of Trustees (2023-present)
- Sharon Blackburn, chief judge, U.S. District Court, Northern District of Alabama (2006-2013); senior status (2015-present)
- Mike Bolin, justice, Alabama Supreme Court (2004 – 2023); Jefferson County Commissioner seat for District 5 (2023-present)
- Houston L. Brown, first African American presiding judge, 10th Circuit, Jefferson County, Alabama (2000-2015); founding member of the Birmingham Committee for Truth and Reconciliation, which honored Angela Davis (2019)
- Charles J. Crist, Jr., governor, state of Florida (2007–11); U.S. House of Representatives (Florida, 2016-2022)
- Stephen Louis Armstrong Dillard, chief judge (2017-2019), Georgia Court of Appeals; Georgia Court of Appeals, judge (2010-present)
- Joel F. Dubina, chief judge (2009-2013), U.S. Court of Appeals, Eleventh Circuit
- Cordell Hull, 47th U.S. secretary of state (1933–44), Nobel Peace Prize winner for his role in establishing the United Nations (1945); longest serving secretary of state
- James E. Horton, presided over the retrial in the “Scottsboro Boys” case in 1933 on the Eighth Circuit Court of Alabama; ruled against the death penalty for Haywood Patterson, for which he was removed from the case.
- Howell Edmunds Jackson, U.S. Supreme Court justice (1893–95)
- Thomas Lemuel Johns, U.S. Secret Service; present during John F. Kennedy assassination (1963)
- Doug Jones, U.S. Senator (Alabama, 2018-2021); Nomination Advisor for Legislative Affairs, appointed by Biden (2022)
- Horace Harmon Lurton, U.S. Supreme Court justice (1909–14); Confederate sergeant major in the Civil War, paroled by Lincoln
- Charles Robert Malone, chief justice, Alabama Supreme Court (2011–13)
- Brady E. Mendheim Jr., justice, Alabama Supreme Court (2018 – present)
- Nina Miglionico, civil rights advocate and first female elected to Birmingham City Council; Birmingham Woman of the Year, 1963
- Kevin C. Newsom, U.S. circuit judge, U.S. Court of Appeals Eleventh Circuit (2017–present)
- Horace Elmo Nichol, chief justice, Georgia Supreme Court (1975–80)
- Robert Ashby Pate, justice, Supreme Court of Palau (2013-2016); helped create first-ever jury trial system on Palau
- Loreta Raulinaityte, permanent representative, Seimas of the Republic of Lithuania to the European Union (2006–2013)
- Martha Roby, U.S. House of Representatives (Alabama, 2011 – 2021); one of the first women elected to Congress from Alabama in regular elections
- Greg Shaw, justice, Alabama Supreme Court (2009 to present)
- J. Griffin Smith, chief justice, Arkansas Supreme Court (1937–55)
Religion
- Charles Billingsley, Christian singer, worship leader, songwriter, author, worship leader at Thomas Road Baptist Church, Lynchburg, Virginia
- Matthew Cook, moderator-elect, Cooperative Baptist Fellowship
- Scott Dawson, founder, Scott Dawson Evangelistic Association; founder, Strength to Stand Conference movement
- Herschel Hobbs, pastor, First Baptist Church, Oklahoma City; president, Southern Baptist Convention (1960s); author; Baptist World Alliance leader
- Richard Lance, executive director, Alabama Baptist Convention
- Wanda Seay Lee, executive director, Women’s Missionary Union
- Russ Levenson, pastor, St. Martin’s Episcopal, Houston, Texas
- Randall Lolley, retired president, Southeastern Baptist Theological Seminary
- Martha C. Myers, M.D., Christian missionary to Yemen
- R. Albert Mohler, president, Southern Baptist Theological Seminary
- Willy Rice, senior pastor, Calvary Baptist Church; president, Southern Baptist Convention Pastor’s Conference
- Ed Stetzer, president, Lifeway Research
- Ralph Douglas West, pastor, The Church Without Walls, Houston, Texas
- J. Robert White, executive director, Georgia Baptist Convention
Sports
- Bobby Bowden, head football coach, Florida State University (1976–2009), national champion (1993, 1999); College Football Hall of Fame (2006)
- Marv Breeding, player, Major League Baseball—Baltimore Orioles, Washington Senators, Los Angeles Dodgers (1961–63)
- Phillip Ervin, first-round draft pick, Cincinnati Reds (2013)
- Cortland Finnegan, player, National Football League—Tennessee Titans, St. Louis Rams, Miami Dolphins (2006–14); Pro Bowl (2009)
- Wendell Magee, player, Major League Baseball—Philadelphia Phillies, Detroit Tigers, Cleveland Indians (1996–2002)
- Jaquiski Tartt, second-round NFL draft pick (highest drafted football player in Samford history, 46th overall pick)—San Francisco 49ers (2015)
- Corey White, player, National Football League—New Orleans Saints (2012–present)
- Alyssa Whitehead, professional soccer player, Sweden (2011–13)
- Nick Williams, player, National Football League—Pittsburgh Steelers (2013–present)